Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - APPENDIX C Dye Calibration and Install Standard Checks; Calibrate Dyes

Dye calibration and install standard
checks
■
Calibrate dyes ...................................................... 192
■
Perform an install run ................................................ 203
Calibrate dyes
Dye calibration compensates for dye emission spectral overlap.
1.0
0.8
0.6
0.4
0.2
0
5
10 15
20
25
30
35
40
result = 0, q = 0.998, c = 6.374
Capillary 1
Figure 20 Dye emission spectra for the J6 (DS-36) dye set showing spectral overlap
System dye sets are available from Thermo Fisher Scientic.
System dye sets
Fragment/HID analysis dye sets Sequence analysis dye sets
• D (DS-30, DS-31)
• E5 (DS-02)
• F (DS-32)
• G5 (DS-33)
• J6 (DS-36)
• J6-T (DS-37)
• E_BigDye
™
Terminator v1.1
• Z_BigDye
™
Terminator v3.1
• Z_BigDye
™
Direct
Custom dye sets are any dyes that are not available from Thermo Fisher Scientic.
Custom dye sets require manual calibration.
C
Overview of
system dye set
and custom dye
set calibration
System versus
custom dyes
192
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide