Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Factory, Auto, and Manual Calibration; Determine if Manual Calibration Is Required

Three types of calibration can occur on the instrument:
• Factory calibration—Default calibration provided with the instrument. It is not
optimized for a specic instrument.
• Manual calibration—Manual procedure performed by the user that provides a
baseline calibration for the instrument on which it is run. Reduces pull-up (false
secondary peaks under a true peak).
• Auto calibration—Automatic adjustment of the baseline calibration to optimally
reduce pull-up (false secondary peaks under a true peak). The instrument
performs an auto calibration for system and custom dyes.
• Sequence analysis dye sets—Do not typically require manual calibration.
• Fragment analysis dye sets—Manual calibration is recommended one time
before use.
Note: With high-quality sample data, it is possible for auto calibration to pass
using the factory calibration.
• HID analysis dye sets—Manual calibration is required for each dye set one time
before use.
To determine if a dye set requires manual calibration, review the calibration history
for the dye set.
In the home screen:
Factory, auto, and
manual
calibration
Determine if
manual
calibration is
required
Appendix C Dye calibration and install standard checks
Calibrate dyes
C
SeqStudio
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Genetic Analyzer Instrument and Software User Guide
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