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Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Workflow: HID Analysis

Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer
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Workflow: HID analysis
Get started
Prepare the instrument
(page 39)
Prepare the samples
(page 37)
Create a plate setup on the instrument
Create or import a plate setup
(page 81)
Enter plate properties
(page 82)
Assign wells: run module, size standard, dye set, and kit
(page 84)
Assign wells: sample name, sample type, and custom fields
(page 86)
Start and monitor a run
On the instrument:
Load the plate or the tube assembly
(page 92)
Select a plate setup and start a run
(page 93)
Monitor a run from the instrument
(page 105)
View and analyze results
“View results on the instrument“ on page 111
Export results from the instrument (sample data files and QC reports)
(page 121)
Analyze data in a secondary analysis software application
HID install run
Item
Description
Components of the install check
reaction
GeneScan
600 LIZ
Size Standard v2.0 (Cat. No. 4408399)
HiDi
Formamide (Cat. No. 4311320 or 4440753)
GlobalFiler
Allelic Ladder from one of the following installation kits:
SeqStudio
HID and Sequencing Installation Kit (Cat. No. A46180)
SeqStudio
HID Installation Kit (Cat. No. A46182)
Appendix D Create and run an HID plate
Workflow: HID analysis
D
SeqStudio
Genetic Analyzer Instrument and Software User Guide
215

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