Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Data Output; Secondary Analysis Software

Data output
SeqStudio
™
Data Collection Software generates an electropherogram (intensity plot)
for each dye that is based on the migration of DNA fragments through the capillaries
during a run.
The format of the sample data les is determined by the type of experiment that is
specied at the time of plate setup.
• Sequencing experiments use basecalling (the algorithms and seings required to
determine the fragment base sequences) and generate an AB1 le.
• Fragment/HID analysis experiments use sizecalling (the algorithms and seings
required to determine the fragment sizes) and generate an FSA le.
The AB1 and FSA le formats can be analyzed by secondary analysis software.
Secondary analysis software
Secondary analysis software is available for desktop computers and on your Connect
cloud-based platform account.
Visit thermosher.com/connect for the latest available secondary analysis
applications.
Note: Data from the SeqStudio
™
Genetic Analyzer may be labeled as "3200" in
secondary analysis software.
Secondary analysis apps on the Connect cloud-based platform
Analysis
App Description
Sequencing Quality Check (QC) module
• Automatically checks sequence trace quality.
• Provides a results summary that is based on quality
parameter settings.
• Auto-flags lower-quality traces for further inspection.
Variant Analysis (VA) module
• Finds variants in samples that are sequenced on Applied
Biosystems
™
genetic analyzers.
• Reports variants at genomic coordinates.
• Allows export of variant calls in standard Variant Call
Format.
Next‑generation Confirmation
(NGC) module
• Confirms next-generation sequencing (NGS) variants
using CE technology.
• Allows visualization of the variants that are detected by
both NGS and CE platforms.
• Allows export of confirmed variants in standard Variant
Call Format.
Chapter 1 Product information
Data output
1
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
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