Item
Description
Volumes of install check components
per reaction
• Size standard—0.4 µL
• Hi‑Di
™
Formamide—9.6 µL
• Allelic ladder—1 µL
Pass/fail criteria for HID J6 install
check
• 343 ladder peaks
• 26 size standard peaks
• Minimum peak height >400 RFU
• Standard deviation of the observed allele fragment sizes for all
4 capillaries: <0.15 base pairs (bp)
HID run module, dye sets, size standard definitions, and kits
Run module
Resolution
range
Approximate
run time
Sizing precision Compatible size standards
HIDAnalysis 60–470 bp
[1]
39 minutes 60–470: <0.15
• GeneScan
™
500 LIZ
™
Size
Standard
[2]
• GeneScan
™
600 LIZ
™
Size
Standard v2.0
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 size
standard sample sized with a third order fit) is ≥1.
[2]
The GeneScan
™
500 LIZ
™
Size Standard was not included in the HID validation of the instrument.
Category
Items
Dye sets
• G5 (DS-33)
• J6 (DS-36)
• J6-T (DS-37)
Size standards
• GS500(-250)LIZ
• GS600_LIZ_(60-460)
• GS600_LIZ_(80-400)
Kits
• Setting of None disables the Marker-
to-Marker pull-up feature ("Kit" is
displayed when None is selected.
• GlobalFiler
™
• GlobalFiler
™
Express
• Huaxia
™
Platinum
• Identifiler
™
Plus
• MiniFiler
™
• NGM Detect
™
• NGM SElect
™
• VeriFiler
™
Express
• VeriFiler
™
Plus
• Yfiler
™
• Yfiler
™
Plus
Appendix D Create and run an HID plate
HID run module, dye sets, size standard definitions, and kits
D
216
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
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