5.
Touch a sample le name, then touch View.
If you select more than one sample le name, the View buon is dimmed.
If the data triggered any quality alerts, a QC alerts screen is displayed.
For information on quality alerts, see:
Click View data to display the trace for the sample. See “Fragment/HID analysis
trace“ on page 113 or “Sequence analysis trace“ on page 115.
6.
Touch and drag the thumbnail view of the analyzed trace (below the trace) to
scroll left or right.
7.
Touch
or to scroll to the raw data or EPT Plot.
Data quality alerts
Quality alert
Description Action
Offscale peaks. Adjust the injection
parameters and/or the sample
concentration.
At least 10 scans have saturated the
CCD camera.
• Reduce the injection voltage or
time.
• Dilute the sample.
No sample was detected. Poor signal‑to‑noise ratio with low
signal detected.
• Verify that the sample volume
follows recommendations in the
user manual.
• Troubleshoot upstream PCR and
sequencing steps.
Sizecalling and basecalling quality alerts
Table 5 Sizecalling quality alerts
Quality alert
Description Action
Sizing quality value is low due to poor size
standard peak quality. Peak height uniformity is
low or the fitting quality in sizing is poor.
Low resolution or poor quality
data is present.
• Re-inject the sample.
• If the problem persists,
check the sample quality.
Sizecaller found broad peak(s) in the size
standard peak(s).
Sizing quality value is in the intermediate range;
check size standard data quality.
The number of size standard peaks detected is
less than what is defined in the size standard.
Size standard definition
includes peaks that are not
present in the sample. Example:
Sample peaks are detected up
to 500 bp, but the size standard
definition includes peak sizes
that are >500 bp.
Use or create a size standard
definition with the appropriate
number of peaks and peak
sizes.
Chapter 8 View and analyze results
View results on the instrument
8
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
119
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