Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Edit Injection Parameters and Re-Inject Samples; Export a Report (QC Report)

Quality alert Description Action
The analysis range is too small. Correct the
analysis range in analysis settings and re-
analyze in secondary analysis software or re-
inject sample.
Various causes.
• Analyze the data in a
secondary analysis
software with a corrected
analysis range.
• Re-inject the sample.
Table 6 Basecalling quality alerts
Quality alert Description Action
Basecalling failed due to poor quality
data.
Poor quality data is present.
• Re-inject the sample.
• If the problem persists, prepare
fresh sample.
• Troubleshoot upstream PCR and
sequencing steps.
You can edit injection parameters and re-inject samples during a run or after a run is
complete.
1.
Access Injection options.
• During a run—Touch Actions4Edit plate.
• After a run—Touch Results.
2.
Touch an injection group, then congure the injection list:
• Touch and drag an injection group to a new location in the injection list.
• Touch Inject rst—Moves the selected injection group to the top of the
injection list.
• Touch Edit and re-inject—Adds replicates or re-injections to the injection list.
You can also modify Run module, Injection time, Injection voltage, Run
time, or Run voltage for these injections.
3.
Touch Done.
Note: The changes are not applied until you touch Done.
This function allows you to export a QC report for the current plate. To export a QC
report for a previously run plate, export a run history (see “Export results from the
instrument (sample data les and QC reports)“ on page 121).
When a run is complete, in the home screen:
1.
Touch Results.
2.
Touch Export report.
3.
Select a storage location.
4.
Navigate to, then select a location, then touch Export.
Edit injection
parameters and
re-inject samples
Export a report
(QC report)
Chapter 8 View and analyze results
View results on the instrument
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SeqStudio
™
Genetic Analyzer Instrument and Software User Guide