8 • DP/SP Troubleshooting
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To troubleshoot:
1. Check all tubing connections for signs of blockage and replace it as
needed. Be careful not to overtighten fittings.
2. As columns age, their backpressure increases. It may be necessary to
compensate for this by increasing the high pressure limit. If the column is
the source of the high backpressure, clean the column. (Refer to the
column manual provided on the Dionex Reference Library CD-ROM
(P/N 053891) for instructions.) If this does not eliminate the problem,
replace the column.
3. Observe a run to see whether the high pressure limit is triggered when
injection occurs; if it is, the injection valve may be the source of the
blockage (i.e., the rotor seal or stator may need to be replaced). Contact
Dionex for assistance.
This error occurs when the cap on the seal wash reservoir is equipped with a
drop sensor and the number of drops of wash solution detected by the sensor
while the peristaltic pump is idle (55 minutes per hour) exceeds the leak
detection threshold.
To troubleshoot:
1. Inspect the piston seals for leakage. Replace the piston seals and O-rings
as needed (see Section 9.5
).
2. Increase the value for the rear-seal leak threshold:
a. In Chromeleon or Chromeleon Xpress, select Command on the
Control menu or press the F8 key.
b. In the Commands dialog box, select the pump name.
c. Select the RearSealLeakCounter command.
d. Enter a new value and click Execute.
The rear-seal leak count is x.x (counted drops) and has
exceeded the limit of y.y (leak detection threshold).
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