29.15 Dosing and Liquid Handling
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
452
￭￭￭￭￭￭￭￭
900 Touch Control
:
Filling time in s
: maximum filling rate in mL/min
: current filling rate in mL/min
: Filling time for one cylinder volume:
￭ 20 s (exchange unit)
￭ 18 s (dosing unit)
The maximum filling rate depends on the cylinder volume of the exchange
unit / dosing unit attached (see chapter 32, page 492). The approximate
filling times (including valve switching) are given in the table below for var-
ious cylinder volumes (exchange unit and dosing unit) at different filling
rates:
Cylinder vol-
ume
Filling time in s
max. 100 mL/min 50 mL/min 10 mL/min
1 mL 24 / – – – –
2 mL – / 22 – – –
5 mL 24 / 22 – – 34
10 mL 24 / 22 – – 64
20 mL 24 / 22 – 28 124
50 mL 24 / 22 34 64 304
Application example
1 L reagent is to be dosed within 1 hour with a 50 mL exchange unit.
How large is the dosing rate?
Conditions:
: Total duration = 60 min
:
Dosing volume = 1,000 mL
:
Filling time = 24 s (assumption: max. filling rate)
:
Cylinder volume = 50 mL
Calculation of the number of filling procedures:
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