Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Page 140

2.
To create a new run module:
a.
Touch a default run module or a user-created run module to use as a
starting point, then touch Copy.
b.
Enter values, then touch Next.
c.
Enter a name, touch Advanced to enter additional seings, then Done.
See “Run module seings“ on page 140 for detailed information.
3.
As needed, touch a run module of interest, then touch Edit or View.
Note: The Edit buon is dimmed if a run is in progress.
Run module settings
Setting
Description
Run module name Name of the module
Capillary temperature (°C) Temperature setting for the capillary array throughout run
Prerun voltage (Volts) Voltage setting for pre-run before sample injection
Prerun time (seconds) Prerun time
Injection voltage (Volts) Voltage for sample injection
Injection time (seconds) Sample injection time
Run voltage (Volts) Final sample electrophoresis separation run voltage
Run ramp duration (seconds) Time required to reach the Run voltage
Note: Data collection does not start until this time elapses.
Run time (seconds) Length of time that data is collected after the Run ramp duration
elapses
Run modules, read lengths, size ranges, and run times
Table 7 Sequencing run modules for standard sequencing
Run module
Contiguous read
length (CRL)
[1]
QV threshold
Approximate run
time
ShortSeq ≥350 QV30 30 minutes
ShortSeq_BDX
MediumSeq ≥500 QV30 45 minutes
MediumSeq_BDX
Chapter 10 Manage the software (instrument)
Manage run settings (instrument)
10
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SeqStudio
™
Genetic Analyzer Instrument and Software User Guide