Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, Glossary.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Glossary-7
R
n
The normalized reporter signal is the cycle-by-cycle ratio of the fluorescence of the
reporter dye and the fluorescence of the passive reference dye in a given well. During
PCR, R
n
increases as the amplification copy number increases until the reaction
approaches a plateau. When using the instrument in its Plate Read mode (end point
analysis), the fluorescence signal is read at a single point in time after the completion
of PCR rather than at intervals during the course of PCR.
run A run describes a collection of fluorescence data carried out by the 7900 instrument
during and/or after cycling of samples in an optical plate or TaqMan
®
Low Density
Array.
session See Analysis Session.
single nucleotide
polymorphism
(SNP)
A single base pair of DNA in the genome that differs between individuals.
singleplex A PCR reaction with a single fluorescent detector per well.
SNP See single nucleotide polymorphism.
spectral bin A portion of the complete range of the wave length measured by the 7900HT
instrument (approximately 500-660 nm).
spectral calibration Spectral calibration occurs from pure dye results obtained from a pure dye run. The
software stores the spectral information for the pure dye standards within a calibration
file located in the SDS directory. After a run, the software extracts each component
dye from the collected data in the pure spectra run file. Because the age and use of the
instrument components can affect pure spectra readings, Applied Biosystems
recommends updating the pure spectra run file once or twice annually depending on
instrument use.
spectral crosstalk The fluorescent contribution of one dye signal to another caused by the overlap of their
spectra.
stack The physical container of a 7900HT instrument into which well plates can be stacked.
There are 5 stacks in all. Four of the five consecutively numbered stacks are used to
hold the sample plates while the fifth is used by the Plate Handler to hold the first set
of analyzed plates. Maximally 20 plates can be placed in each stack. The instrument
can process maximally eighty plates in one batch.
study A study in the SDS Enterprise Database option is a named collection of zero or more
AD or RQ analysis sessions. A study in the SDS software main application is an '.sdm'
plate document file that contains one or more '.sds' relative quantification plate
document files.
task A setting applied to the detectors within a well of a plate document that determines
how the data collected from the well during analysis are used. The task selections
available differ depending on the plate's assay type.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English