Hologic Panther System - Overview; Technology Overview; Target Capture

Panther System
®
Operator’s Manual
AW-17791-001 Rev. 002 (EN)
25
Overview
Overview
This section contains the following information:
l Technology Overview
l PantherSystem Overview
l Panther System Software Overview
Technology Overview
The Aptima assays are nucleic acid amplification tests that detect DNA or RNA from a
variety of approved specimen types and collection devices according to the appropriate
package insert.
Aptima assays may utilize the following proprietary technologies:
l Target Capture—Isolation and purification of nucleic acid from the sample.
l Transcription-Mediated Amplification (TMA
®
)—Amplification of target nucleic acid
to produce multiple copies of RNA for easier detection.
l Hybridization Protection Assay (HPA)—Detection of the amplified nucleic acid
using light-emitting nucleic acid probes.
l Dual Kinetic Assay (DKA)—Analysis of kinetic chemiluminescent data to
differentiate the presence of two signals in a single tube.
l Real-Time Assays—Assays that measure emission of fluorescence during the TMA
process.
l Internal Controls for Aptima Assays—May be used to control for nucleic acid
capture, amplification, and detection, as well as operator or instrument error.
Target Capture
The Target Capture process isolates nucleic acid of the target virus and purifies nucleic
acid for amplification.