Panther System
®
Operator’s Manual
AW-17791-001 Rev. 002 (EN)
26
Overview
Figure 2. Target Capture
1. An MTU is picked from the MTU Input Queue and placed in the sample dispense
station.
a. wTCR (that is kept in suspension by rotary mixing) is pipetted into the MTU tubes.
A single disposable tip is used to dispense TCR into all five reaction tubes within
an MTU.
b. Cooled sample is dispensed into each tube using individual pipette tips. Each
time a sample is dispensed, the pipettor verifies that both the wTCR and sample
have been dispensed accurately.
c. The distributor moves the MTU to the sample mix station where the fluids are
mixed.
d. The MTU moves to the High Temperature Incubator for target capture. At this
step, oligonucleotides called "capture oligos" hybridize to the target nucleic acid
sequence in the specimen. Cell lysis may also occur during this step for some
assays.
e. The MTU moves to the Transition Incubator, where the capture oligo/target
nucleic acid complexes hybridize to magnetic particles (beads) by their tail
sequences.
f. The MTU is cooled in a Chiller Ramp and moves into a parking slot where
magnets pre-capture magnetic TCR beads to the sides of the MTU wall.
2. Once the wTCR beads are captured, the MTU moves into the Magnetic Wash Station
Module (Mag Wash) where the captured target is separated from the residual fluids.
The Mag Wash goes through a process of injecting wash solution, mixing, capturing
particles, and aspirating residual solution to remove sample and wTCR residues.
3. The vacuum system is used for aspiration, ensuring optimal airflow and minimizing
aerosols. Following the final aspiration, oil is dispensed into the MTU to minimize
evaporation during the rest of the assay process.
4. When the Mag Wash cycle is complete, the purified target nucleic acid is ready to be
amplified by Transcription-Mediated Amplification.
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