Thermo Scientific Evolution 201 - Nucleic Acid

4
Life Science Applications
Nucleic Acid
138 Evolution 200 Series User Guide Thermo Scientific
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Nucleic Acid
Overview
See Unique Screen Features for information about the results the application calculates. See
Nucleic Acid Concentration Calculations for information about the calculations.
Method
Analytical
Wavelength
(Quant)
Reference
Wavelength
Start
(Scan)
Stop
(Scan)
Bandwidth
Integration
Time (sec)
(Fixed, Scan)
Data
Interval
(Scan)
Scan
Speed
(nm/min)
Nucleic Acid 340 220 350 1.0 1.0, 0.08 0.50 375
Nucleic Acid
Labels
340 220 850 1.0 1.0, 0.16 1.00 375
Protein A280 340 220 850 1.0 1.0, 0.08 0.5 375
Proteins & Labels 340 220 850 1.0 1.0, 0.16 1.0 375
Pierce BCA 562 340 750 450 1.0 1.0, 0.12 1.00 500
Protein Bradford 595 340 750 450 1.0 1.0, 0.12 1.00 500
Pierce Modified
Lowry
750 405 900 600 1.0 1.5, 0.12 1.00 500
Pierce 660 nm
Protein Assay
660 340 850 550 1.0 1.5, 0.12 1.00 500
Protein Biuret 550 340 750 450 1.0 1.0, 0.12 1.00 500
Contents
• Overview
• Nucleic Acid Concentration Calculations
• Application Settings
• Unique Screen Features
• Making Nucleic Acid Measurements
• Oligo Calculator
Use the Nucleic Acid application to measure nucleic acid
samples for concentration and purity.