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Life Science Applications
Nucleic Acid Labels
156 Evolution 200 Series User Guide Thermo Scientific
Instrument Tab for Nucleic Acid Labels
Accessories Tab for Nucleic Acid Labels
Unique Screen Features
Related Topics
Overview
Dye/Chromophore Editor
Application Settings
Making Nucleic Acid Labels Measurements
Oligo Calculator
The right pane in Measure Nucleic Acid Labels
includes:
Feature Description
Type Type of analysis to perform. To the right is the factor value from the
Type tab.
Conc. Concentration based on absorbance at 260 nm, the default or
user-defined extinction coefficient, and the specified pathlength. (The
extinction coefficient is reported for a 10 mm pathlength.) Beer’s law
is used to calculate the nucleic acid concentration (see Nucleic Acid
Concentration Calculations).
The displayed A260 value is baseline corrected. The A260 value
actually used to calculate the concentration takes into account the
appropriate dye correction factors and any absorbance correction due
to the selected analysis correction wavelength.
A260 Absorbance at 260 nm.
260/280 Ratio of sample absorbance at 260 nm and 280 nm, used to assess the
purity of DNA and RNA. A ratio of ~1.8 is generally accepted as
“pure” for DNA, ~2.0 for RNA. If either ratio is appreciably lower,
protein, phenol or other contaminants that absorb strongly near
280 nm may be present. See Nucleic Acid Purity Measurements for
more information.
Dye(s) Conc. Concentration of the user-selected dye(s).
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