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Life Science Applications
Pierce Modified Lowry
Thermo Scientific Evolution 200 Series User Guide 201
To measure a sample again, right-click its row in the results table and choose Remeasure.
After the remeasurement, the previous information for the sample is crossed out (but not
removed from the table).
To copy the data on the Standard Curve(s) tab or Run Chart tab, right-click the plot and
choose Copy to Clipboard.
Related Topics
Overview
Application Settings
Bradford Standard Curves
Data Display
Pierce Modified Lowry
Overview
This assay is an alternative method for determining protein concentration based on the widely
used and cited Lowry procedure for protein quantitation. Like the other colorimetric assays,
the Lowry assay requires generating a standard curve before measuring sample proteins.
The Pierce Modified Lowry procedure involves reaction of protein with cupric sulfate in
alkaline solution, resulting in formation of tetradentate copper-protein complexes. The
Folin-Ciocalteu Reagent is effectively reduced in proportion to the chelated
copper-complexes, resulting in a water-soluble blue product that is measured at 650 nm and
normalized at 405 nm. Preformulated reagents, used in the assay, are available in kit form
from numerous manufacturers.
Contents
• Overview
• Application Settings
• Lowry Standard Curves
• Making Pierce Modified Lowry Assay Measurements
The Pierce Modified Lowry assay measures absorbance at
750 nm to determine concentration.
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