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Life Science Applications
Nucleic Acid
140 Evolution 200 Series User Guide Thermo Scientific
The 260/230 ratio assay determines the purity of nucleic acids in the presence of phenol,
which absorbs strongly at 230 nm.
A ratio of approximately 1.8 is generally accepted as “pure” for DNA, approximately 2.0 for
RNA. If either ratio is appreciably lower, protein, phenol or other contaminants that absorb
strongly near 280 nm may be present.
The 260/230 values for a “pure” nucleic acid are often higher than the respective 260/280
values and are commonly in the range 1.8 to 2.2. If the ratio is appreciably lower, copurified
contaminants may be present.
Application Settings
Type Tab for Nucleic Acid
Measurement Tab for Nucleic Acid
Instrument Tab for Nucleic Acid
Accessories Tab for Nucleic Acid
Samples Tab for Nucleic Acid
Related Topics
Overview
Nucleic Acid Concentration Calculations
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
Type Tab for Nucleic Acid
To set data acquisition parameters for a Nucleic Acid
workbook, click Settings to access:
Click Settings in Nucleic Acid to display the Type tab in
the right pane.
Feature Description
Name Application name.
Description (optional) Description of the template.