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Life Science Applications
Nucleic Acid
Thermo Scientific Evolution 200 Series User Guide 141
Related Topics
Measurement Tab for Nucleic Acid
Instrument Tab for Nucleic Acid
Accessories Tab for Nucleic Acid
Samples Tab for Nucleic Acid
Measurement Tab for Nucleic Acid
Nucleic Acid Type Type of analysis to perform.
ssDNA is for single-stranded DNA.
Oligo DNA and Oligo RNA use the appropriate extinction
coefficient based on entered DNA or RNA base sequence. The
entry will appear on the Oligo Calculator tab.
For Custom, enter a factor (extinction coefficient) by which to
multiply the measured absorbance. The factor used for other types
cannot be changed.
Pathlength Distance the light travels through the sample.
Feature Description
Click Settings in Nucleic Acid to display the
Measurement tab in the right pane.
Feature Description
Analysis wavelength Wavelength(s) to use for the analysis.
Correction Specifies bichromatic normalization of the absorbance data.
To use uncorrected data, select None. Without baseline correction,
spectra may be offset from the baseline. If this offset is significant, the
calculated protein concentration may be higher than the true value.
Single point subtracts the sample absorbance at the entered
wavelength from the absorbance at every data point.
Sloping baseline draws a line between the data points at the entered
wavelengths, finds its absorbance value at the analytical wavelength,
and subtracts it from the measured absorbance.
Component name Component to quantify.
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