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Life Science Applications
Nucleic Acid
146 Evolution 200 Series User Guide Thermo Scientific
Related Topics
Overview
Nucleic Acid Concentration Calculations
Application Settings
Making Nucleic Acid Measurements
Oligo Calculator
Making Nucleic Acid Measurements
While performing this procedure, click here for information about features to the right of the
spectral display.
Y To measure nucleic acids
1. Click Measure Nucleic Acid in Nucleic Acid.
2. Click Measure.
3. Follow the instructions that appear.
Use the appropriate buffer for the blank, generally the same buffer used to prepare or
dissolve the sample. The buffer should be the same pH and of a similar ionic strength as
the sample solution.
A280 Absorbance at 280 nm.
260/280 Ratio of sample absorbance at 260 nm and 280 nm, used to assess the
purity of DNA and RNA. A ratio of about 1.8 is generally accepted as
“pure” for DNA, about 2.0 for RNA. If either ratio is appreciably
lower, protein, phenol or other contaminants that absorb strongly
near 280 nm may be present. See Nucleic Acid Purity Measurements
for more information.
260/230 Ratio of absorbance at 260 nm and 230 nm, a secondary measure of
nucleic acid purity.
Feature Description
Note To start an analysis immediately after viewing or changing the template settings,
click the Measure action button instead and skip to step 3.
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