Thermo Scientific Evolution 201 - Making Protein A280 Measurements; Unique Screen Features

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Life Science Applications
Protein A280
Thermo Scientific Evolution 200 Series User Guide 167
Accessories Tab for Protein A280
Unique Screen Features
Related Topics
Overview
Application Settings
Making Protein A280 Measurements
Making Protein A280 Measurements
While performing this procedure, click here for information about features to the right of the
spectral display.
Y To make a Protein A280 measurement
1. Click Measure Protein A280 in Protein A280.
2. Click Measure.
The right pane in Measure Protein A280 may include:
Feature Description
Type Type of analysis to perform.
ε/1000 and Molecular
Weight (kDa)
ε/1000 and molecular weight for the Other protein (ε &
MW) sample type.
Ext. Coeff, ε 1% (L/g-cm) Extinction coefficient and ε 1% for the Other protein (ε
1%) sample type.
Conc. Concentration based on absorbance at 280 nm, after
correction is applied, and the selected extinction coefficient.
A280 Absorbance at 280 nm for the protein sample being
measured.
260/280 Ratio of absorbance at 260 nm and 280 nm.
Note To start an analysis immediately after viewing or changing the template settings,
click the Measure action button instead and skip to step 3.