Thermo Scientific Evolution 201 - Nucleic Acid Concentration Calculations; Overview

4
Life Science Applications
Nucleic Acid
Thermo Scientific Evolution 200 Series User Guide 139
Related Topics
Nucleic Acid Concentration Calculations
Application Settings
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
Nucleic Acid Concentration Calculations
The Nucleic Acid application is used for determining the concentration and purity of nucleic
acid samples. The nucleic acid concentration is determined by multiplying the absorbance at
260 nm by a factor (default, 50). This factor is essentially the extinction coefficient in units of
ng-cm/ml. Using this coefficient gives the manipulated equation
where c is the nucleic acid concentration in ng/microliter, A is the absorbance in AU, ε is the
wavelength-dependent extinction coefficient in ng-cm/microliter, and is the pathlength in
centimeters.
The generally accepted extinction coefficients for nucleic acids are:
Related Topics
Overview
Application Settings
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
Nucleic Acid Purity Measurements
The 260/280 nm ratio assay determines the purity of nucleic acids based on the 260/280
ratio.
Coefficient Value
Double-stranded DNA 50 ng-cm/μl
Single-stranded DNA 33 ng-cm/μl
RNA 40 ng-cm/μl
l
)/ *(
A
= c ε