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Life Science Applications
Nucleic Acid Labels
Thermo Scientific Evolution 200 Series User Guide 159
Oligo Calculator
Y To use Oligo Calculator
1. Enter a base sequence on the Oligo Calculator tab.
More:
Use:
• The buttons below the Base Sequence box.
• The A, C, G, T and U keys on the keyboard.
• Copy and paste a base sequence (A, C, G, T and U only) from another application.
To clear the sequence, click Clear. Individual bases can be deleted only manually.
2. Select the nucleic acid type to analyze.
3. Select the degree of phosphorylation if applicable: mono-phosphate for DNA; mono- or
tri-phosphate for RNA.
4. Select Double-stranded if applicable. The complementary base sequence will be
included in the analysis.
5. Enter the molecular weights of any additions to the base sequence in the Modification
box.
The calculated results appear:
Use Oligo Calculator to calculate molecular weight,
extinction coefficients, concentration factors and
melting points for specific nucleic acid sequences.
Clicking this task button displays:
Tab Description
Oligo Calculator For entering sequences of interest and selecting appropriate sample type
variables.
Melting Displays the calculated melting points of a DNA strand. Available only
for DNA sequences.
Result Description
Molecular weight Calculated base sequence molecular weight.
Molar extinction
coefficient
Calculated 260 nm extinction coefficient in ng-cm/microliter.
Concentration factor Factor, based on the extinction coefficient, used to calculate the
concentration of the base sequence.
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