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Life Science Applications
Protein A280
160 Evolution 200 Series User Guide Thermo Scientific
Y To calculate the melting point of a DNA sequence
1. Enter the base sequence on the Oligo Calculator tab.
2. Enter appropriate values on the Melting tab:
The calculated results appear:
Protein A280
Overview
Number of bases Number of bases in the entered sequence.
% GC Percentage of the total number of bases made up by guanine
and cytosine.
Parameter Description
Oligo molarity Concentration of the Oligo in molar units.
Cation molarity Concentration of cations in the sample.
% Formamide Percentage concentration of formamide in the sample.
Result Description
Salt-adjusted Calculated melting point of the base sequence, corrected for the
concentration of cation in the sample. This method does not
account for the effect of interaction between neighboring bases.
Nearest-neighbor Melting point of the base sequence when the effect of interaction
between neighboring bases is taken into account.
Result Description
Contents
• Overview
• Application Settings
• Unique Screen Features
• Making Protein A280 Measurements
The Protein A280 method is used to quantify proteins on
the basis of their absorbance at 280 nm.
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