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Life Science Applications
Pierce 660 nm Protein Assay
Thermo Scientific Evolution 200 Series User Guide 219
Related Topics
Type Tab for Pierce 660 nm Protein Assay
Measurement Tab for Pierce 660 nm Protein Assay
Standards Tab for Pierce 660 nm Protein Assay
Instrument Tab for Pierce 660 nm Protein Assay
Accessories Tab for Pierce 660 nm Protein Assay
Pierce 660 nm Assay Workbooks
When opened, the Pierce 660 nm Protein Assay application may prompt for a new standard
curve, requiring the creation of a new workbook. If a previously saved workbook is used, all
concentration calculations for newly measured samples will be based on the saved curve
archived in the workbook.
Related Topics
Overview
Application Settings
Standard Curves
Making Pierce 660 nm Protein Assay Measurements
Standard Curves
A standard curve is required for colorimetric protein analysis.
• A standard curve can be generated using two or more standards.
• The multipoint curve capability allows multiple replicates for each standards. Standards
can be run in any order; however, best practice dictates that the standards be measured
from the lowest concentration to the highest.
• Determine whether or not to use a standard when analyzing samples against the standard
curve.
• Establishing a new standard curve requires creating a new workbook.
• If a previously saved workbook is used, all concentration calculations for newly measured
samples will be based on the standard absorbance values saved in the workbook.
Note Each workbook will archive only one standard curve.
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