Thermo Scientific Evolution 201 - Accessories Tab for Protein A280; Application Settings; Instrument Tab for Protein A280; Measurement Tab for Protein A280

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Life Science Applications
Protein A280
Thermo Scientific Evolution 200 Series User Guide 161
Tyrosine, tryptophan, phenylalanine and to a small extent lysine and Cys-Cys disulphide
bonds have an absorbance peak around 280 nm. This method does not require generation of
a standard curve and is ready for protein sample quantitation at software startup. Colorimetric
assays such as Pierce BCA, Pierce 660 nm, Bradford and Lowry are more commonly used for
uncharacterized protein solutions and cell lysates.
The Protein A280 application displays the UV spectrum, measures the protein’s absorbance at
280 nm (A280), and calculates the concentration (mg/ml).
Related Topics
Application Settings
Unique Screen Features
Making Protein A280 Measurements
Application Settings
Type Tab for Protein A280
Measurement Tab for Protein A280
Instrument Tab for Protein A280
Accessories Tab for Protein A280
Samples Tab for Protein A280
Related Topics
Overview
Unique Screen Features
Making Protein A280 Measurements
Type Tab for Protein A280
To set data acquisition parameters for a Protein A280
workbook, click Settings to access:
Click Settings in Protein A280 to display the Type tab in
the right pane.
Feature Description
Name Application name.
Description (optional) Description of the template.