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Life Science Applications
Pierce BCA
Thermo Scientific Evolution 200 Series User Guide 187
Procedure
Y To make a Pierce BCA measurement
1. Click Measure Pierce BCA in Pierce BCA.
2. If standards need to be acquired, click Measure. If standards have already been acquired,
skip to step 7 to acquire sample data.
3. Follow the instructions that appear.
Use the appropriate buffer for the blank. See the reagent directions for details.
4. When the standards information appears, modify it if desired.
More:
If Standard averaging on the Standards tab in Settings was set to Duplicate, “D” at the
end of a standard name indicates the second measurement to be made of the standard. If
Sample averaging was set to Triplicate, “D” and “T” at the end of standard names indicate
the second and third measurements to be made, respectively.
To enter previously saved standards information, use Import Standards.
If Calculate from weight/volume was selected on the Standards tab, enter the weight and
volume for each standard in the table.
To remove a standard, select it, right-click the table and choose Clear Standard. To
remove all the standards, choose Clear Table.
To save standards information for later use, use Export Standards.
5. Click Continue.
6. Follow the instructions that appear, installing the specified standards.
Note Standards diluted from a stock standard must cover the expected range of the
unknown samples. Protein concentrations are not extrapolated beyond measured
standard concentrations.
Note To start an analysis immediately after viewing or changing the template settings,
click the Measure action button instead and skip to step 3.
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