Thermo Scientific Evolution 201 - Accessories

4
Life Science Applications
Pierce 660 nm Protein Assay
Thermo Scientific Evolution 200 Series User Guide 221
To remove a standard, select it, right-click the table and choose Clear Standard. To
remove all the standards, choose Clear Table.
To save standards information for later use, use Export Standards.
5. Click Continue.
6. Follow the instructions that appear, installing the specified standards.
The Standard Curve(s) tab displays the resulting standard curve (or curves). Specify the
standards to use for the curve (or curves) by selecting Yes or No in the Use column in the
table.
More:
The table contains the columns of information specified on the Configuration tab in
Reports. Examples include sample identification, user name, and the results of replicate,
duplicate or triplicate measurements and their standard deviation.
If Standard averaging was set to Duplicate, “D” at the end of a standard name indicates
the second measurement. If Standard averaging was set to Triplicate, “D” and “T”
indicate the second and third measurements, respectively.
To permanently remove an acquired standard from the analysis, right-click it and choose
Remove. Its information is crossed out but not removed from the table.
The Data tab displays the acquired data (a fixed data point or scan spectrum) for the
standard selected in the table. To access commands for customizing the display, including
adding annotation, right-click the data. See Data Display for more information.
7. Click Measure.
If a standard curve is not calibrated...
• Try selecting a different curve fit type.
• Try remeasuring a standard using the correct standard material:
select the listed standard, right-click the table and choose
Remeasure.
• Try changing the setting of Minimum r
2
on the Standards tab and
acquire the standards again in a new workbook.
This is only an indicator that the required minimum number of points
have been established for the selected curve fit type. It does not validate
the integrity of the curve. For example, additional standards may be
required to cover the expected assay concentration range.