Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, Glossary.fm
Glossary-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
SDS 7900HT
Document (*.sds)
A file used by the SDS software to store plate documentation locally on a computer
hard drive. A plate document is a virtual representation of a consumable (plate) used
to contain samples and reagents during a sequence detection run. During the run, the
software uses the plate document to record the operation of the instrument (thermal
cycling and data collection parameters), organize and store the data gathered during
the PCR process, store the of the run data, and record analysis and display options.
SDS 7900HT
Tem p lat e
Document (*.sdt)
Plate document templates have the same format as the SDS 7900HT Documents
(*.sds) but do not store raw data or bar codes.
absolute
quantification (AQ)
The objective of an absolute quantification experiment is to accurately determine the
absolute quantity of a single nucleic acid target sequence within an unknown sample.
The results of an absolute quantification experiment are reported in the same units of
measure as the standard used to make them.
allele One of the variant forms of a gene at a particular locus or location on a chromosome
allelic
discrimination (AD)
The process by which two variants of a single nucleic acid sequence present in a
population of individuals' DNA samples is determined. Single nucleotide
polymorphisms (SNPs) comprise the most common variants that are assayed using
TaqMan reagents.
amplicon A PCR amplified section of DNA
Amplification plot The Amplification plot displays data from real-time PCR for gene expression assays
after signal normalization and multi-component analysis. The Amplification plot
appears only in plate documents containing analyzed data from real-time runs. It is
a 2D plot of normalized reporter fluorescence (for ∆R
n
) values versus the cycle
number. The ∆R
n
versus cycle Amplification plot is used for calculation threshold
cycle (C
T
).
analysis session A set of analysis results derived from a single raw data set associated with a single
plate document analyzed by the SDS software. The analysis session is persisted in the
database and can be retrieved by the SDS software. Multiple analysis sessions can be
created from the same plate document, however each analysis session must have a
unique name. An analysis session does not have to be associated with a Study;
however, if it has such a relationship, it can be associated with at most one Study at
any given time.
annealing Binding a probe to the DNA or cDNA during the PCR process
Application
Program Interface
(API)
A set of routines, protocols, and tools for building software applications. A good API
makes it easier for a programmer to develop a program by providing all the building
blocks necessary to link the elements of the various subcomponents of the system.
assay type With reference to the SDS 2.2 Enterprise Option software, an assay type is one of the
following: allelic discrimination, absolute quantification, or relative quantification.
auto increment
value
Method setting that increases or decreases the value for any PCR set-point parameter
(time or temperature) by a fixed amount every cycle.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English