Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, Glossary.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide Glossary-1
Glossary
.sdd The file extension of an ABI PRISM SDS Detector file for SDS software for the
7900HT instrument.
.sdm The file extension of a SDS 7900HT Multiple Plate Document (*.sdm) for SDS
software for the 7900HT instrument.
.sds The file extension of a SDS 7900HT Document (*.sds) for SDS software for the
7900HT instrument.
.sdt The file extension of a SDS 7900HT Template Document (*.sdt) for SDS software for
the 7900HT instrument.
∆C
T
The difference between the threshold cycle of a sample assay and the threshold cycle
of the corresponding endogenous reference: ∆C
T
= C
T
(target) - C
T
- C
T
(endogenous
control).
∆∆C
T
For a given cDNA target, the difference between the average ∆C
T
value of a target
sample and the average ∆C
T
for the corresponding calibrator sample:
∆∆C
T
(test sample) = Avg∆C
T
(test sample) - Avg∆C
T
(calibrator sample). This value
is used to calculate expression fold value by the equation: Expression fold value =
2
−∆∆C
T
∆R
n
The normalized reporter signal minus the baseline signal. The baseline is established
in the first few cycles of PCR. Like R
n
, ∆R
n
is not a fixed value but increases during
PCR when the amplicon copy number increases until the reaction approaches a
plateau.
5′ nuclease assay The 5′ (five prime) nuclease assay in combination with the TaqMan
®
probes serves as
the basis for real-time polymerase chain reaction (PCR) technology on the 7900HT
instrument. See “Fundamentals of the 5´ Nuclease Assay” on page D-2 for a detailed
description of the reaction.
ABI P
RISM SDS
Detector file (*.sdd)
The SDS detector file is a tab-delimited flat file that is used to transfer detector
information to and from the SDS software using the Export and Import functions of
the Detector Manager software. The file contains: (a) a header
“SDS2.2.1[GDF[Global Detector File”, (b) tab-delimited data for each detector (allele
Name, the name of the reporter dye, the name of the quencher dye, a description, a
comment, the owner, the creation date and time, the modification date and time, and
the color used to code the detector in graphical displays of the SDS software).
SDS 7900HT
Multiple Plate
Document (*.sdm)
Multiple plate documents are used for the analysis of relative quantification data.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English