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Thermo Scientific Evolution 201 - Page 239

Thermo Scientific Evolution 201
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4
Life Science Applications
Protein Biuret
Thermo Scientific Evolution 200 Series User Guide 225
Related Topics
Measurement Tab for Protein Biuret
Standards Tab for Protein Biuret
Instrument Tab for Protein Biuret
Accessories Tab for Protein Biuret
Samples Tab for Protein Biuret
Measurement Tab for Protein Biuret
Standard curve Type of quantitative analysis used.
Pathlength Distance the light travels through the sample.
Feature Description
Click Settings in Protein Biuret to display the
Measurement tab in the right pane.
Feature Description
Analysis wavelength Wavelength(s) to use for the analysis.
Correction Specifies bichromatic normalization of the absorbance data.
To use uncorrected data, select None. Without baseline correction,
spectra may be offset from the baseline. If this offset is significant, the
calculated protein concentration may be higher than the true value.
Single point subtracts the sample absorbance at the entered
wavelength from the absorbance at every data point.
Sloping baseline draws a line between the data points at the entered
wavelengths, finds its absorbance value at the analytical wavelength,
and subtracts it from the measured absorbance.
Component name Component to quantify.

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