Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Preparing TaqMan Low Density Arrays for Use
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-21
2. Remove the sealed Low Density Array by grasping its sides and lifting it off the
sealer’s insert plate.
Note: In the middle of the sealer’s insert plate, there is a thumb slot to help you
easily access one side of the Low Density Array.
3. Inspect the Low Density Array for proper sealing. The indentations from the
stylus assembly should match up with the Low Density Array’s main channels.
If the indentations do not match up or if the foil is in any way damaged, do not
use the Low Density Array.
4. Return the carriage to its starting position on the base of the sealer.
IMPORTANT! Do not move the carriage back before removing the Low Density
Array.
Trimming Off the
Fill Reservoirs
1. Using scissors, trim the fill reservoirs from the Low Density Array. Use the edge
of the Low Density Array’s carrier as a guide.
PHYSICAL INJURY HAZARD. Take care when trimming
off the fill reservoirs. Use scissors rather than razor blades or other unprotected
cutting devices.
The Low Density Array is now ready to be run on the 7900HT instrument.
2. Choose from the following. If performing:
– Absolute or Relative Quantification, or Dissociation Curve Analysis
Run the plate as explained in “Running the Plate” on page 4-25.
– Allelic Discrimination
a. Load the plate onto a designated thermal cycler and perform the PCR.
b. Briefly centrifuge the plate to draw the reactions to the bottom of the wells and
to eliminate any air bubbles that may have formed during thermal cycling.
c. Run the plate as explained in “Running the Plate” on page 4-25.
GR2175
Thumb slot
GR2168

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English