DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Exporting Plate Document Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-17
4.
In the
Export
drop-down list, select the type of data you would like to export.
•
Background Spectra
– The exported file contains the fluorescence readings
for each well from the background component used to analyze the run
•
Clipped
– The exported file contains the average R
n
and
∆
R
n
of the last
three data points collected during the extension phase of each cycle repetition
for each well
•
Dissociation Curve –
The exported file contains:
Temperature Data – For each well in use on the plate, the file displays the
calculated temperature of the wells during each data collection reading of the
temperature ramp.
Raw Data – For each well in use on the plate, the file displays the R
n
of the
well during each data collection reading of the temperature ramp.
Derivative Data – For each well in use on the plate, the file displays the first
derivative data during each data collection reading of the temperature ramp.
•
Multicomponent –
The exported file contains:
– Amounts the calculated dye components in a single well throughout all
stages of the PCR that were labeled with data collection icons
– Pure spectra component data
– Calculated inverse matrix
– Singular values of the inverse matrix
•
Pure Spectra
– The exported file contains the fluorescence readings for each
well from the pure spectra calibration component used to analyze the run
•
Raw Spectra
– The exported file contains the unmodified fluorescence
readings taken for each spectral bin during the course of the run
• When exported, the software creates a directory and saves each the raw
spectra data for each well in a separate *.txt file.
•
Results Table
– The exported file contains the contents of the table pane of
an analyzed plate document
Note:
The contents of the exported data vary depending on the type of plate
document.
•
Setup Table
– The exported file contains the contents of the table pane of a
plate document before analysis The contents of the exported data varies
depending on the type of plate document used to produce it. See page A-4 for
a detailed description of the Setup Table file.
5. To export data from:
• All wells of the plate document – Select the All Wells radio button.
• Selected wells of the plate grid – Select the Selected Wells radio button.
6. In the files of type drop-down list, select the appropriate format for the exported
data.
7. Click the File name text box, and enter a name for the exported file.
8. Click .
The software saves the exported data to the designated location.
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