Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Exporting Plate Document Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-17
4. In the Export drop-down list, select the type of data you would like to export.
• Background Spectra – The exported file contains the fluorescence readings
for each well from the background component used to analyze the run
•
Clipped – The exported file contains the average R
n
and ∆ R
n
of the last
three data points collected during the extension phase of each cycle repetition
for each well
•
Dissociation Curve – The exported file contains:
Temperature Data – For each well in use on the plate, the file displays the
calculated temperature of the wells during each data collection reading of the
temperature ramp.
Raw Data – For each well in use on the plate, the file displays the R
n
of the
well during each data collection reading of the temperature ramp.
Derivative Data – For each well in use on the plate, the file displays the first
derivative data during each data collection reading of the temperature ramp.
•
Multicomponent – The exported file contains:
– Amounts the calculated dye components in a single well throughout all
stages of the PCR that were labeled with data collection icons
– Pure spectra component data
– Calculated inverse matrix
– Singular values of the inverse matrix
•
Pure Spectra – The exported file contains the fluorescence readings for each
well from the pure spectra calibration component used to analyze the run
•
Raw Spectra – The exported file contains the unmodified fluorescence
readings taken for each spectral bin during the course of the run
• When exported, the software creates a directory and saves each the raw
spectra data for each well in a separate *.txt file.
•
Results Table – The exported file contains the contents of the table pane of
an analyzed plate document
Note: The contents of the exported data vary depending on the type of plate
document.
•
Setup Table – The exported file contains the contents of the table pane of a
plate document before analysis The contents of the exported data varies
depending on the type of plate document used to produce it. See page A-4 for
a detailed description of the Setup Table file.
5. To export data from:
• All wells of the plate document – Select the All Wells radio button.
• Selected wells of the plate grid – Select the Selected Wells radio button.
6. In the files of type drop-down list, select the appropriate format for the exported
data.
7. Click the File name text box, and enter a name for the exported file.
8. Click .
The software saves the exported data to the designated location.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English