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Applied Biosystems 7900HT User Manual

Applied Biosystems 7900HT
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DRAFT
September 1, 2004 11:40 am, 4351684ATOC.fm
iv Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Managing Sequence Detection System Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-17
Section 1.3 SDS Enterprise Database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-21
About the SDS Enterprise Database Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-22
About the SDS Enterprise Database Software Suite . . . . . . . . . . . . . . . . . . . . . . . . . . 1-25
Database Modules for Large-Scale Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-25
Database Management Utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-26
Database Design and Information Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-27
Supporting API Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-28
Chapter 2 Getting Started
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Powering On the 7900HT Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Using the SDS Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Basic Software Skills Tutorial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
Lesson 1: Using Plate Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12
Lesson 2: Viewing and Resizing Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-21
Lesson 3: Using the Plate Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-23
Lesson 4: Using the Hand-Held Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . 2-27
Lesson 5: Using Contextual Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-28
Lesson 6: Using Keyboard Shortcuts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-28
Using SDS Plate Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-29
Chapter 3 Preparing a Run
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Workflow Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Quick Review: Powering On the 7900HT Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Step 1 – Creating a Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
Step 2 – Applying Detectors and Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Step 3 – Configuring the Plate Document with Tasks . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
Step 4 – Setting the Passive Reference and Omitting Wells . . . . . . . . . . . . . . . . . . . . 3-18
Step 5 – Programming the Plate Document Method . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
Step 6 – Saving the Plate Document as a Template . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
Step 7 – Creating a Plate Document from the Template . . . . . . . . . . . . . . . . . . . . . . . 3-26
Step 8 – Applying Sample and Plate Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-28
Step 9 – Running the Plate on the 7900HT Instrument . . . . . . . . . . . . . . . . . . . . . . . . 3-29

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Applied Biosystems 7900HT Specifications

General IconGeneral
BrandApplied Biosystems
Model7900HT
CategoryLaboratory Equipment
LanguageEnglish

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