Observation Possible cause Recommended action
Normalization calibration failed
(continued)
One or more wells produced
spectra that deviate significantly
from the rest of the array card.
1.
Locate the wells with the abnormal signal
(see “View the calibration images” on
page 101).
2.
Rotate the array card 180°, then perform
the calibration again.
3.
Determine the location of the contaminated
wells again. If the positions of the wells
identified in the steps above are:
•
Identical: The sample block is
contaminated. Decontaminate the
sample block.
•
Reversed: The normalization array
card is contaminated. Discard the
consumable, then perform the
normalization calibration using a new
normalization array card.
4.
If the calibration fails again, contact
Support.
Perform verification using RNase P plat
es
Verification should be performed under the following circumstances:
•
After installation and before first use of the instrument and the block
•
After performing instrument and block calibrations
•
As needed to confirm instrument and block performance
The instrument and block require valid ROI/uniformity, background, and dye calibrations to perform
verification.
Chapter 7 C
alibrate and verify instrument performance
Perform verification using RNase P plates
7
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
103
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