Calibration workflow
Perform an ROI/uniformity calibration
You are automatically prompted to perform background calibration.
▼
Perform a background calibration
Perform any time that ROI/uniformity calibrations are current.
▼
Perform system dye calibrations
Perform any time that ROI/uniformity and background calibrations are current.
▼
(Optional ) Perform custom dye calibrations (see “Calibrate custom dyes” on page 113)
Perform any time that ROI/uniformity and background calibrations are current.
Prepare a calibration plate
Required materials to prepare calibration plates
Note: We recommend calibrating with all Spectral Dye Calibrations Plates available for your block
configuration even if you are not using all the dyes in the plates. This is recommended for optimal
system performance.
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Calibration plates
ROI/Uniformity plate
Background calibration plate
Dye calibration plates
Equipment and consumables
Centrifuge with plate adapter; buckets cleaned before use MLS
Powder‑free gloves MLS
Safety glasses MLS
Chapter 7 Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations for plate blocks
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QuantStudio
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6 Pro Real-Time PCR System and QuantStudio
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7 Pro Real-Time PCR System User Guide
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