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Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Calibrate custom dyes

Thermo Scientific Applied Biosystems QuantStudio 7 Pro
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3.
Load the TaqMan
Array Card into the instrument.
CAUTION! The instrument should be used by tr
ained operators who have been warned of the
moving parts hazard.
4.
T
ap Start.
5.
When the run is complete and the screen displays Complete, tap View results to confirm the
status of the run.
Verification status Action
Passed Instrument is ready for use.
Failed See “Troubleshoot verification failur
e” on page 107.
6.
In the RNase P V
erification Status screen, tap one of the following options.
Option Description
Accept results Save the results to the instrument.
Reject results Deletes the RNase P verification r
esults.
Export results Exports the results.
7.
Unload the T
aqMan
Array Card from the instrument.
CAUTION! PHY
SICAL INJURY HAZARD. During instrument operation, the TaqMan
Array
Card temperature can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smar
t Help if the instrument does not eject the plate (see “Request technical support
with Smart Help” on page 172).
The status of the RNase P verification is displayed on the S
tatus and Reminders screen (see “View the
calibration status on the instrument” on page 87).
IMPORTANT! An RNase P T
aqMan
Array Card can only be used one time. Do not reuse the RNase P
TaqMan
Array Card.
Calibrate custom dyes
Overview of custom dyes
The instrument can run assays designed with custom dyes. The following items are defined as cust
om
dyes:
Dyes that are not manufactured by Thermo Fisher Scientific.
Dyes or formulations of dyes that are not system dyes for the instrument.
Chapter 7 C
alibrate and verify instrument performance
Calibrate custom dyes
7
QuantStudio
6 Pro Real-Time PCR System and QuantStudio
7 Pro Real-Time PCR System User Guide
113

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