Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Perform ROI;uniformity, background, dye, and normalization calibrations for the TaqMan Array Card block

Perform ROI/uniformity, background, dye, and
normalization calibrations for the TaqMan
™
Array Card
block
To calibrate the plate blocks, see “Perform ROI/uniformity, background, and dye calibrations for plate
blocks” on page 89.
This section covers procedures specific to the TaqMan
™
Array Card block. See the following sections
for additional information:
•
Transfer calibration results (page 94)
•
Transfer data files from calibrations (page 94)
•
Troubleshoot calibration failure (page 95)
To troubleshoot normalization calibration, see “Troubleshoot normalization calibration failure” on
page 102.
•
Identify contamination (page 95)
Calibration workflow
Perform an ROI/uniformity calibration
You are automatically prompted to perform background calibration.
▼
Perform a background calibration
Perform any time that ROI/uniformity calibrations are current.
▼
Perform system dye calibrations
Perform any time that ROI/uniformity and background calibrations are current.
▼
Perform normalization calibration
Perform after a dye calibration.
▼
(Optional) Perform custom dye calibrations (see “Calibrate custom dyes” on page 113)
Perform any time that ROI/uniformity and background calibrations are current.
Chapter 7 Calibrate and verify instrument performance
Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
™
Array Card block
7
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
97