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Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Features of each instrument; Instrument filters and supported dyes

Thermo Scientific Applied Biosystems QuantStudio 7 Pro
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Smart Help to request technical support or instrument service directly from the instrument
Barcode scanner for tracking plates or TaqMan
Array Cards
Features of each instrument
Feature
QuantStudio
6 Pro System QuantStudio
7 Pro System
Filter set
[1]
Coupled five-color filter set Decoupled six-by-six color filter set
VeriFlex
Zones
[2]
Three VeriFlex
Zones Six VeriFlex
Zones
TaqMan
Array Card
block
Available
Security, Auditing, and
Esignature (SAE) v2.0
module
Available
[3]
[1]
S
eeSystem dyes” on page 16.
[2]
See “VeriFlex
Zones” on page 18.
[3]
See Chapter 9, “Use the instrument with the Security, Auditing, and Esignature (SAE) v2.0 module”.
Instrument filt
ers and supported dyes
System dyes
The QuantStudio
6 Pro Real-Time PCR System has a coupled five-color filter set.
The QuantStudio
7 Pro Real-Time PCR System has a decoupled six-by-six color filter set.
For more information about available spectral dye calibration kits, contact Support.
Peak filter Color
Filter wavelength (nm)
[1]
System dyes Example custom dyes
Excitation Emission
x1-m1 Blue 470 ± 15 520 ± 15
FAM
dye, SYBR
GREEN
dye
SYT09
x2-m2 Green 520 ± 10 558 ± 11 VIC
dye
JOE
dye, HEX
dye,
TET
dye
[2]
x3-m3 Yellow 550 ± 11 586 ± 10
NED
dye, TAMRA
dye, ABY
dye
Cy
®
3 dye
x4-m4 Orange 580 ± 10 623 ± 14 ROX
dye, JUN
dye Texas Red
dye
x5-m5 Red 640 ± 10 682 ± 14
MUSTANG PURPLE
dye, Cy
®
5
LIZ
dye
x6-m6 Deep-Red 662 ± 10 711 ± 12 None
[3]
Cy
®
5.5 dye
[1]
T
he central wavelengths are the optimized wavelengths.
[2]
The HEX
dye and TET
dye from Thermo Fisher Scientific fall within the emission wavelength range of the system, therefore they
can be added and adapted for use on the instrument.
[3]
This filter set currently does not support any dyes supplied by Thermo Fisher Scientific.
Chapter 1 P
roduct information
Instrument hardware description
1
16
QuantStudio
6 Pro Real-Time PCR System and QuantStudio
7 Pro Real-Time PCR System User Guide

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