5.
When the run is complete and the screen displays Complete, tap View results to confirm the
status of the run.
Verification status Action
Passed Instrument is ready for use.
Failed See “Troubleshoot verification failure” on page 107.
6.
In the RNase P V
erification Status screen, tap one of the following options.
Option Description
Accept results Save the results to the instrument.
Reject results Deletes the RNase P verification r
esults.
Export results Exports the results.
7.
Unload the plat
e from the instrument.
CAUTION! PHY
SICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smar
t Help if the instrument does not eject the plate (see “Request technical support
with Smart Help” on page 172).
The status of the RNase P verification is displayed on the S
tatus and Reminders screen (see “View the
calibration status on the instrument” on page 87).
IMPORTANT! RNase P plat
es can only be used one time. Do not reuse the RNase P plate.
Troubleshoot verification failur
e
Observation Possible cause Recommended action
Verification failed
The plate was improperly
pr
epared.
Ensure the following:
•
The correct plate was used for the
verification performed.
•
The plate was properly thawed.
•
The plate was properly centrifuged.
•
The plate was properly sealed.
Open the data file for verification in the desktop
software or the Connect platform to view the
flags and the troubleshooting details for the
failed wells.
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P plates
7
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
107
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