Thermo Scientific Applied Biosystems QuantStudio 7 Pro - APPENDIX C Detailed procedures for preparation of a TaqMan Array Card; Guidelines for preparation of a card

Detailed procedures for preparation
of a TaqMan
™
Array Card
Guidelines for preparation of a card
•
Keep the card protected from light and stored as indicated until ready for use. Excessive exposure
to light may aect the fluorescent probes of the dried-down assays in the card.
•
Before removing the card from its packaging:
–
Prepare each sample-specific PCR reaction mix.
–
Allow the card to reach room temperature.
•
Load each fill reservoir with 100 µL of sample-specific PCR reaction mix.
–
Each fill reservoir contains a single sample as determined by the card layout.
–
The 100–µL volume ensures adequate filling of each reaction well. Volumes smaller than
100 µL result in insuciently filled cards.
•
Do not allow the micropipette tip to contact the coated foil beneath the fill port.
C
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QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide