Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Perform verification using RNase P with a TaqMan Array Card

Observation Possible cause Recommended action
Verification failed
(continued)
The plate is damaged or
contaminated.
Check for damage, improper plate seal, or
contamination.
Order a replacement plate. Use Smart Help
if the replacement plate fails (see “Request
technical support with Smart Help” on
page 172).
High fluorescence signal
The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered in
the Method tab.
Signals that exceed the
limit of normal fluorescence
can indicate fluorescent
contaminants on the plate or on
the sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate and
run the experiment again.
Identify the location of contamination on the
plate or sample block.
1.
Obtain or prepare a background plate (see
“Create a background plate (optional)” on
page 96).
2.
Follow the procedures to identify
contamination (see “Identify contamination”
on page 95).
Verification failed but plate is
undamaged
The incorrect plate was used for
verification.
Use the correct RNase P plate for verification.
The plate was improperly
prepared.
Repeat the verification with a new properly
prepared plate.
Note: Each RNase P plate can only be used
once.
Open the data file for verification in the desktop
software or the Connect platform to view the
flags and troubleshooting details for failed wells.
If the verification fails again, order a replacement
plate. Use Smart Help if the replacement plate
fails (see “Request technical support with Smart
Help” on page 172).
Perform verification using RNase P with a TaqMan
™
Array
Card
Verification should be performed under the following circumstances:
•
After installation and before first use of the instrument and the block
•
After performing instrument and block calibrations
•
As needed to confirm instrument and block performance
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P with a TaqMan
™
Array Card
7
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QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide