Thermo Scientific Applied Biosystems QuantStudio 7 Pro - TaqMan Array Card diagram

•
Load the card with PCR reaction mix before centrifuging the card.
During centrifugation, the PCR reaction mix resuspends the dried-down assays in each well of the
card. Adding sample after centrifuging disrupts the assay layout of the card.
•
Run the card within the time allowed by the PCR master mix. See the user guide for the assay or
the master mix.
•
If the card is not run immediately, protect it from light and store at 2-8°C.
TaqMan
™
Array Card diagram
A TaqMan
™
Array Card includes 8 fill reservoirs and 384 reaction wells.
1
2
3
4
5
1
Fill reservoirs (8 total)
2
Fill reservoir strip
3
Array card carrier
4
Array card barcode
5
Reaction well (384 total)
1
2
2
1
Foil
2
Array card feet
The fill reservoir includes a fill port and a vent port. Use the fill port to load PCR reaction mix into the
card.
Appendix C Detailed procedures for preparation of a TaqMan
™
Array Card
TaqMan
™
Array Card diagram
C
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
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