Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Prepare an Rnase P Taqman Array Card

Prepare an RNase P TaqMan
™
Array Card
Required materials to prepare an RNase P TaqMan
™
Array Card
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Calibration kit
ViiA
™
7 Array Card RNaseP Verification Kit, includes the following
components:
•
TaqMan
™
Array Card
•
Eight (8) vials of RNase P detection reagents, one for each port
in the TaqMan
™
Array Card. Each vial contains the following
reagents:
–
PCR master mix
–
RNase P primers
–
A FAM
™
dye-labeled probe
–
A known concentration of human genomic DNA template
4432265
Equipment and consumables
Centrifuge with custom buckets and card holders, one of the following:
•
Sorvall
™
centrifuge
•
Multifuge
™
centrifuge
•
Megafuge
™
centrifuge
Contact your local sales oce
TaqMan
™
Array Card Sealer Contact your local sales oce
Blank balance TaqMan
™
Array Cards Contact your local sales oce
Pipette, 200 µL with tips MLS
Permanent marker or pen MLS
Powder‑free gloves MLS
Safety glasses MLS
Chapter 7 Calibrate and verify instrument performance
Perform verification using RNase P with a TaqMan
™
Array Card
7
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QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide