Perform calibrations
1.
In the home screen tap (Settings)4Maintenance and service4Calibration.
The Calibration screen is displayed.
2.
In the Calibration screen, select a calibration type.
Calibration type Tap
ROI/uniformity ROI and uniformity
Background Background
Dye System dye
Normalization Instrument normalization
Note: Y
ou will be prompted to perform a background calibration after a ROI/Uniformity calibration.
3.
(S
ystem dye calibration only) Select the dye to calibrate.
•
FAM
•
VIC
•
ROX
4.
(Normalization only) Select the reporter dye and passive reference to calibrate.
•
FAM/ROX
•
VIC/ROX
5.
Follow the instructions on the screen to start the calibration.
6.
Load the TaqMan
™
Array Card into the instrument.
See “Load a TaqMan
™
Array Card into the instrument” on page 52.
CAUTION! The instrument should be used by tr
ained operators who have been warned of the
moving parts hazard.
7.
T
ap Start.
When the ROI/Uniformity run is complete, you will be prompted to perform a background
calibration.
When the Background calibration is complete, the Calibration Status will display. It will indicate
whether the calibration passed or failed.
Chapter 7 C
alibrate and verify instrument performance
Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
™
Array Card block
7
100
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
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