Filters
Wavelength
(nm)
1 2
3
4
5
x1-m1 x2-m2
x3-m3
x4-m4
x5-m5 x6-m6
Emission
Spectra
1
x1-m1—FAM
™
dye, S
YBR GREEN
™
dye
2
x2-m2—VIC
™
dye
3
x3-m3—ABY
™
dye, NED
™
dye, Cy
®
3, T
AMRA
™
dye
4
x4-m4—JUN
™
dye, RO
X
™
dye, Texas Red
™
dye
5
x5-m5—Cy
®
5, MUS
TANG PURPLE
™
dye
Custom dyes
The instrument can run assays designed with custom dyes. Custom dyes include the following options:
•
Dyes that are not supplied by Thermo Fisher Scientific.
•
Dyes or formulations of dyes that are not system dyes for the instrument.
To use a custom dye on the instrument, review the following requirements.
•
Calibrate the instrument for the custom dye (see “Calibrate custom dyes” on page 113).
•
Ensure that the custom dye excites between 455–672 nm and emits between 505–723 nm.
•
Select a custom dye that does not overlap with other dyes used in the run (see “System dyes” on
page 16).
•
The custom dye for use in calibration must be attached to the 5' end of a short DNA
oligonucleotide consisting of the first two bases of the probe sequence without a quencher at
the 3' end.
Chapter 1 P
roduct information
Instrument hardware description
1
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
17
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