Thermo Scientific Applied Biosystems QuantStudio 7 Pro - Page 8

■
CHAPTER 7 Calibrate and verify instrument performance ..................... 84
Instrument and block calibration ................................................. 84
Calibration and verification schedule ............................................. 85
Calibration descriptions ......................................................... 86
View the calibration status and set reminders ...................................... 87
View the calibration status on the instrument .................................. 87
Set calibration frequency and reminders on the instrument ...................... 88
View calibration status and set reminders on the Connect platform .............. 88
Review calibration status ................................................... 89
Export a calibration report ....................................................... 89
Perform ROI/uniformity, background, and dye calibrations for plate blocks ............ 89
Calibration workflow ....................................................... 90
Prepare a calibration plate .................................................. 90
Perform calibrations ........................................................ 91
View the calibration images ................................................. 93
Transfer calibration results .................................................. 94
Transfer calibration data files ................................................ 94
Troubleshoot calibration failure .............................................. 95
Identify contamination ...................................................... 95
Create a background plate (optional) ......................................... 96
Perform ROI/uniformity, background, dye, and normalization calibrations for the
TaqMan
™
Array Card block .................................................... 97
Calibration workflow ....................................................... 97
Prepare a calibration TaqMan
™
Array Card .................................... 98
Perform calibrations ....................................................... 100
View the calibration images ................................................ 101
Troubleshoot normalization calibration failure ................................ 102
Perform verification using RNase P plates ........................................ 103
Instrument verification description .......................................... 104
RNase P instrument verification plate ....................................... 104
Performance specifications pass criteria ..................................... 105
Prepare an RNase P plate ................................................. 105
Perform RNase P verification ............................................... 106
Troubleshoot verification failure ............................................. 107
Perform verification using RNase P with a TaqMan
™
Array Card ..................... 108
RNase P instrument verification TaqMan
™
Array Card ......................... 109
Prepare an RNase P TaqMan
™
Array Card ................................... 110
Perform RNase P verification ............................................... 112
Calibrate custom dyes ......................................................... 113
Overview of custom dyes .................................................. 113
Use a dilution series to determine an optimal custom dye concentration ......... 114
Calibrate the custom dye .................................................. 117
Calibrate for a custom melt curve run ............................................ 120
Contents
8
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide