Note: You will be prompted to perform a background calibration after a ROI/Uniformity calibration.
3.
(S
ystem dye calibration only) Select the calibration plate.
4.
Follow the instructions on the screen to start the calibration.
5.
Load the plate into the instrument.
CAUTION! The instrument should be used by tr
ained operators who have been warned of the
moving parts hazard.
6.
T
ap Start.
When the ROI/Uniformity run is complete, you will be prompted to perform a background
calibration.
When the Background calibration is complete, the Calibration Status will display. It will indicate
whether the calibration passed or failed.
7.
(For calibrations that pass) Select an option.
Option Description
Details View the calibration images.
Transfer EDS Transfer the calibration file t
o Connect, a USB drive, or a network drive.
Reject results The calibration file is deleted.
Accept results The calibration file is saved.
If the results are accepted and the calibration file is saved, pr
evious calibration files associated with
the combination of the instrument and the block will no longer be used.
8.
(For calibrations that fail) Select an option.
Option Description
Details View the calibration images.
Transfer EDS Transfer the calibration file t
o Connect, a USB drive, or a network drive (recommended
for troubleshooting).
Close The calibration file is deleted.
See “Troubleshoot calibration failure” on page 95.
9.
Unload the plat
e from the instrument.
CAUTION! PHY
SICAL INJURY HAZARD. During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Note: Use Smar
t Help if the instrument does not eject the plate (see “Request technical support
with Smart Help” on page 172).
Chapter 7 Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations for plate blocks
7
92
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
To Next Page
Loading...
