Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-28 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Gene Expression
Settings for Study
Settings
The settings in this group box determine how the software handles the endogenous
control data and RQ Min/Max confidence values for your study. These settings are
crucial to the analysis and are set once for the study
.
Figure 6-11 Gene Expression Settings for Study Settings
• Calibrator Sample – Displays the name of the sample that the software uses as
the calibrator for the study.
• Endogenous Control Detector – Specifies the name of the detector that the
software will use as the endogenous control for the study.
• Control Type – The Multiplexed/Non-Multiplexed option buttons specify how
the software will use the data from the wells labeled with the endogenous
control data.
• RQ Min/Max Confidence – Specifies the confidence value that the software
will use to calculate the standard error of the mean expression level (RQMax
and RQMin values) for the samples in the study.
• Export Individual ∆ C
T
s – Instructs the software to export the ∆ C
T
values of
individual samples in the analysis instead of the average
∆ C
T
values for the
replicate group.
Endogenous Control Detector
drop-down list
RQ Min/Max Confidence drop-down list
Control Type option buttons
Calibrator Sample drop-down list

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English