Applied Biosystems 7900HT - Page 263

DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Verifying Instrument Performance
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-31
Purpose of
TGF-β Runs
The TaqMan
®
Low Density 7900HT Installation Array (TGF-β card) is an
experiment run to verify the performance of the 7900HT instrument for use with
TaqMan Low Density Arrays. The TGF-β card is preloaded with the reagents
necessary for the detection and quantification of genomic copies of the TGF-β gene.
Each well contains preloaded TGF-β primers and FAM MGB dye-labeled probe.
Materials
Required
RNase P Runs
• Appropriate TaqMan RNase P Instrument Verification Plate (see Table 7-2 on
page 7-30)
• Centrifuge, with plate adaptor
TGF-β Runs
•TGF-β card
•TaqMan
®
2✕ Universal PCR Master Mix
• Water, DNase/RNase-free, deionized
• 2-mL microtube, sterile
• Centrifuge
• Microfluidic card sealer
Taq Ma n
®
RNase P
Instrument Verification
Plate
(PN 4310982, standard
96-well plate)
Table 7-2 Configurations of the TaqMan RNase P Instrument Verification Plates
TaqMan RNase P
Instrument Verification
Plate
Sample Configuration
GR2108
Unknown 1
5000
NTC STD 1250 STD 2500
STD 5000 STD 10000 STD 20000
Unknown 2
10000