Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Chapter 7 Maintaining the Instrument
7-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Preparing the
Verification Plate
or TGF-β Card
Preparing a TaqMan RNase P Instrument Verification Plate
1. Remove the TaqMan RNase P Instrument Verification Plate from the freezer
and allow it to thaw to room temperature.
2. Briefly centrifuge the TaqMan RNase P Instrument Verification Plate.
3. Continue with “Preparing the Plate Document” on page 7-33.
Preparing a TGF-β Card
4. Remove the TGF-β card from the refrigerator and allow it to warm to room
temperature.
5. In a sterile 2-mL tube, prepare the following PCR reaction mixture:
6. Tap the tube gently to mix.
7. Pipette 100 µL of the reaction mix into each of the eight fill reservoirs.
8. Continue with “Preparing the Plate Document” on page 7-33.
Reagent Volume (µL)
Taq M a n 2 ✕ Universal PCR Master Mix 500
Human cDNA 50
RNase/DNase-free water 450
Total Volume
1000

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English